You Position: Home > Paper
Connections between GABAergic neurons and Met-enkephalin, Leu-enkepha li n, β-endorphin or dynorphin immunoreactive terminals in the periaqueductal gra y of rat
( views:159, downloads:5 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 20
- DOI:
- 10.3321/j.issn:1000-2790.2001.20.001
- Key Word:
- 水管周灰质;γ-氨基丁酸;阿片样物质;显微镜检查,共焦;荧光抗体技术;免疫组织化学;大鼠
Abstract： 目的观察中脑导水管周围灰质(PAG)内γ-氨基丁酸(GABA)阳性神经元与甲硫氨酸-脑啡肽(Met-ENK)、亮氨酸-脑啡肽(Leu-ENK)、β-内啡肽(β-END)和强啡肽(Dyn) 阳性终末的联系. 方法免疫荧光组织化学双重染色,染色结果在激光共聚焦扫描显微镜下观察. 结果可见直径较小的GABA阳性神经元主要散在分布于PAG的内侧区和腹外侧区. GABA阳性终末在PAG各区的密度均较高. 以小型为主的少量Met-ENK,Leu-ENK和Dyn阳性神经元见于PAG的各区,但未见β-END阳性胞体. Met-ENK, Leu-ENK, β-END和Dyn阳性终末也主要分布于PAG 的内侧区和腹外侧区. Met-ENK, Leu-ENK, β-END和Dyn阳性终末围绕在GABA阳性神经元的胞体或树突的周围并形成密切接触. 结论 [ HTSS〗PAG内含内源性阿片样物质(Opiate-like substances, OLS)的阳性终末与GABA能神经元形成密切接触,提示可能对GABA能神经元的活动具有调控作用.
- [1]A J, Beitz.The midbrain periaqueductal gray in the rat. I. Nuclear volume, cell number, density, orientation, and regional subdivisions.[J].1985,237(4).
- [2]H L, Fields,M M, Heinricher,P, Mason.Neurotransmitters in nociceptive modulatory circuits.[J].1991,14.
- [3]Vaughan, C. W.,Ingram, S. L..How opioids inhibit GABA-mediated neurotransmission.[J].1997,390(6660).
- [4]J L, Moreau,H L, Fields.Evidence for GABA involvement in midbrain control of medullary neurons that modulate nociceptive transmission.[J].1986,397(1).
- [5]Y T, Xuan,Y S, Shi,Z F, Zhou,etc.Studies on the mesolimbic loop of antinociception--II. A serotonin-enkephalin interaction in the nucleus accumbens.[J].1986,19(2).
- [6]周仲福,谢国玺,韩济生.P物质在家兔中脑导水管周围灰质内通过释放脑啡肽引起镇痛[J].1985,(1).
- [7]Vaughan CW,Christie MJ.Presynaptic inhibitory action of opioids on synaptic transmission in the rat periaqueductal grey in vitro.[J].1997,498(Pt.2).
- [8]张安中,曾大允,张玲妹,等.针刺镇痛时家兔中央灰质推挽灌流液中内啡肽含量的变化[J].1979,(9).
- [9]A, Depaulis,M M, Morgan,J C, Liebeskind.GABAergic modulation of the analgesic effects of morphine microinjected in the ventral periaqueductal gray matter of the rat.[J].1987,436(2).