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Regulation factors of telomerase in HL-60 cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 19
- DOI:
- 10.3321/j.issn:1000-2790.2001.19.005
- Key Word:
- 端粒酶;急性髓细胞白血病;HL-60细胞
Abstract： 目的探讨HL-60细胞端粒酶活性的调控因素. 方法用脂质体介导法将LXSN-wt-p53逆转录病毒质粒导入人髓性白血病细胞系HL-60中,用c-myc,bcl -2基因反义脱氧寡核苷酸作用HL-60细胞,用全反式维甲酸(ATRA)诱导HL-60细胞分化后,采用TRAP-ELISA-PAGE法测定端粒酶活性,观察野生型p53基因、c-myc,bcl-2,全反式维甲酸(ATRA)对HL-60细胞端粒酶活性的影响. 结果野生型p53基因不影响HL -60细胞的端粒酶活性;bcl-2及c-myc反义寡核苷酸可下调HL-60细胞的端粒酶活性;ATRA在诱导HL-60细胞分化的同时,可使HL-60细胞的端粒酶活性降低. 结论 HL-60细胞端粒酶活性受到c-myc,bcl-2,全反式维甲酸(ATRA)的调节.
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