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Cloning of genes related to pregnancy induced hypertension syndrome
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITRAY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2001.17.005
- Key Word:
- 高血压;妊娠并发症;胎盘;基因克隆;PCR;减法杂交
Abstract： 目的应用妊娠高血压综合征(妊高征)患者胎盘研究其发病相关基因 . 方法利用基于PCR的改良消减杂交技术,首先建立了该病相关的cDNA消减文库. 随机筛选出20个含有差异表达片段的克隆,经过反向点杂交,弃掉6个假阳性克隆,确定14个克隆为妊高征发病相关基因. 结果经过序列测定和同源性比较(BLAST),发现11个为已知基因,其中1号基因为necdin家族基因;3个为新基因. 这3个新基因已完成了全部插入片段的序列测定,并登录GenBank,登录号为AF232216,AF232217,AF233648. 结论妊高征患者胎盘基因表达谱和正常妊娠胎盘相比,发生改变. 这可能与妊高征的发病有关.
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