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Mitomycin C acts on collagen synthesis of human Tenon's capsule fibrob lasts
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITRAY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2001.17.002
- Key Word:
- 丝裂霉素C;人结膜囊成纤维细胞;胶原合成;细胞培养中图号;R776.1
Abstract： 目的观察丝裂霉素C(MMC)对培养的人结膜囊成纤维细胞胶原合成的影响. 方法用3H-脯氨酸掺入和原位杂交法观察MMC对培养人结膜囊成纤维细胞胶原合成活性的作用. 结果 0.4 g*L-1 MMC作用5 min,可使结膜囊成纤维细胞对3 H-脯氨酸的摄取减少55%以上(P<0 .05),并降低了Ⅰ型前胶原mRNA原位杂交信号. 结论临床青光眼滤过手术中使用的MMC剂量与作用时间,可减少成纤维细胞胶原的合成,有利于青光眼滤过手术成功率的提高.
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