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Differential regulation of sodium pump α-subunit isoform gene by oua bain and digoxin in rat liver
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2001.16.003
- Key Word:
- 哇巴因;地高辛;肝;Na(+)-K(+)-交换ATP酶;高血 压;基因
Abstract： 目的观察哇巴因与地高辛对大鼠肝脏钠泵基因表达的影响. 方法分别给大鼠注射哇巴因(20 μg*kg-1*d-1)、地高辛(32 μg*kg-1*d-1) 和生理盐水(1 mL*kg-1*d-1), 观察给药后 6 wk大鼠血压的动态变化;并分别应用分子生物学RT-PCR及免疫组织化学技术,探讨各组大鼠肝脏钠泵α1, α2及α3亚单位mRNA 及蛋白水平基因表达的改变. 结果给予哇巴因2 wk后大鼠血压开始升高, 至6 wk时明显高于生理盐水组(17.7±1.2) vs (15.4±1.1) kPa, P<0.01), 而实验过程中地高辛组血压与对照组比较差异不明显. 无论是在mRNA水平还是在蛋白水平, 哇巴因对大鼠肝脏钠泵α1亚单位表达无影响,而地高辛使α1亚单位表达增强;两组大鼠α2亚单位表达均无改变;哇巴因使α3亚单位蛋白水平表达减弱,而mRNA水平增强,地高辛组大鼠肝脏钠泵α3亚单位无论在mRNA水平及蛋白水平表达均增强. 结论哇巴因在高血压发病中可能起着重要作用;哇巴因与地高辛可导致不同的钠泵基因表达改变,为进一步揭示内源性哇巴因生理与病理作用以及洋地黄类药物药理与毒理作用的分子机制提供了理论及实验依据.
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