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Inactivity mechanism and retrieval of the antigenic activity of BCL-6 in paraffin sections
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 15
- DOI:
- 10.3321/j.issn:1000-2790.2001.15.003
- Key Word:
- 抗原修复;免疫组织化学;BCL-6;淋巴组织增生
Abstract： 目的探讨石蜡切片中BCL-6抗原失活机制及修复的最佳方法. 方法淋巴组织反应性增生11例石蜡切片分别用二乙胺四乙酸(EDTA)、柠檬酸、碳酸钠及去离子水4种与钙离子结合力不同的修复液,结合高压、微波和煮沸3种不同方法修复后,比较检出的BCL-6阳性率及强度. 结果 BCL-6阳性率由高至低依次为:EDTA 0.73>柠檬酸0.45 (P<0.01)>碳酸钠0.12 (P<0.01),去离子水组无1例阳性. 高压修复阳性率0.55>微波修复0.28和煮沸修复0.12 (P<0.05). 不同修复液与高压结合修复检出BCL-6阳性率及强度:EDTA与柠檬酸阳性率分别为1,0.82(P>0.05),高于碳酸钠组(0.36)和去离子水组(0)(P<0.01). EDTA组强阳性占0.82,显著高于其他各组(P<0.01). 结论 EDTA结合高压是修复BCL-6抗原的理想方法,钙离子的参与可能是甲醛固定后BCL-6抗原失活的原因之一.
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