Abstract： AIM　To observe the effect of electroacupuncture stimulation of zusanli on the expression of Gas and EGFR in gastric mucosa in order to probe mechanisms of acupuncture. METHODS　75 SD rats were randomized into 5 groups: Control group, non-point group, zusanli group, sanyinjiao group and unite point group. All rats were experimented on consciousness. Immunohistochemical staining by means of SABC or SP technique was used to observe the expression, location and distribution of Gas and EGFR in gastric mucosa. Meanwhile, gastric mucosa cells were specially stained with AgNOR. RESUITS　EGFR and Gas in gastric mucous tissue of the zusanli group after EA showed a positive expression significantly higher than those of the control and the non-acupoint group. The expressions of Gas and EGFR were enhanced significantly (66.7% and 73.3%, respectively) in zusanli group. The expression of Gas in gastric mucous tissue were enhanced significantly (46.7%), but the expression of EGFR kept stable in Sanyinjiao group. The expression of Gas was enhanced as significantly as that of EGFR in zu-san groups. Besides, nuclear argentaffin particles that were specially stained with AgNOR didn't change significantly. CONCLUSION　Electroacupuncture stimulation of zusanli can enhance the expression rate of EGFR and Gas in gastric mucoas, but exert no effects on the proliferation of gastric mucous cells.