Abstract: AIM To observe the expression of the selected a ntibodies from the constructed phage display library. METHODS The expressive vectors of solubl e Fab fragment were constructed and the expression of the antibodies was observed through SDS-PAG E and western blotting. The affinity of Fab fragment and HBsAg was also shown by ELISA. RESULTS Expressions of antibodies under the reductive and non-reduc tive conditions were detected as we thought. CONCLUSION The results of antibodies' e xpressions are available for the further research.