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Expression of human anti-HBsAg Fab fragment

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Author:
No author available
Journal Title:
JOURNAL OF THE FORTH MILITARY MEDICAL UNIVERSITY
Issue:
7
DOI:
10.3321/j.issn:1000-2790.2001.07.001
Key Word:
噬菌体抗体;Fab段;电泳,聚丙烯胺凝胶;免疫印 迹法

Abstract: AIM To observe the expression of the selected a ntibodies from the constructed phage display library. METHODS The expressive vectors of solubl e Fab fragment were constructed and the expression of the antibodies was observed through SDS-PAG E and western blotting. The affinity of Fab fragment and HBsAg was also shown by ELISA. RESULTS Expressions of antibodies under the reductive and non-reduc tive conditions were detected as we thought. CONCLUSION The results of antibodies' e xpressions are available for the further research.

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