Investigation of genic expression of TGF-β1 in process of osteoporotic fracture healing in rats
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 6
- DOI:
- 10.3321/j.issn:1000-2790.2001.06.005
- Key Word:
- 骨质疏松;骨折;转化生长因子β;核酸杂交
Abstract： AIM To assess the changes of gene expression of the transformgrowth factors-β1 in the process of the healing of the osteoporotic fracture in ovariectomized female SD rats. METHODS Ovariectomized female SD rats were used as model of type I osteoporosis and osteoporotic fracture and the changes of gene expression of the transforming growth factors-β1 were detected in the process of the healing of the osteoporotic fracture through in situ and blot hybridization. RESULTS There was no difference of the cellular localization in TGF-β1 expression between the processes of the normal and osteoporotic bone healing by in situ hybridization; The pattern of the gene expression of TGF-β1 mRNA was similar to the normal in the process of the osteoporotic bone healing by dot blot hybridization. CONCLUSION Expression of TGF-β1 mRNA is not related to the pathophysiological changes in the process of osteoporotic bone healing.
- [1]B, McKibbin.The biology of fracture healing in long bones.[J].1978,60-B(2).
- [2]M, Centrella,T L, McCarthy,E, Canalis.Skeletal tissue and transforming growth factor beta.[J].1988,2.
- [3]Pamela Gehron Robey,Marian F. Young,Kathleen C. Flanders,etc.Osteoblasts Synthesize and Respond to Transforming Growth Factor-Type β (TGF-β) in vitro[J].1987,105(1).
- [4]M, Centrella,T L, McCarthy,E, Canalis.Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone.[J].1987,262.
- [5]M E, Joyce,S, Jingushi,M E, Bolander.Transforming growth factor-beta in the regulation of fracture repair.[J].1990,21.
- [6]Pfeilschifter J,Seyedin S M,Mundy G R.Transforming growth factor beta inhibits bone resorption in fetal rat long bone cultures.[J].1988.
- [7]A H, Tashjian,E F, Voelkel,M, Lazzaro,etc.Alpha and beta human transforming growth factors stimulate prostaglandin production and bone resorption in cultured mouse calvaria.[J].1985,82.
- [8]M E, Joyce,A B, Roberts,M B, Sporn,etc.Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur.[J].1990,110.