Influence of natural antikeratin autoantibodies on cell cycle of human keratinocytes cultured in vitro
- Author:
- No author available
- Journal Title:
- Journal of Forth Military Medical University
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2001.05.001
- Key Word:
- 抗角蛋白自身抗体;亲和层析;人角质形成细胞;细胞 周期
Abstract： AIM To study the influence of natural antikeratin autoantibod ies (AK autoAbs) on cell cycle of human keratinocytes cultured in vitro. METHODS Natural antikeratin autoantibodies were purified from pooled human serum with affinity chromatograph y, and purity analysis conducted with sodium dodecyl sulphate-polyacrylamide gel electrophore sis (SDS-PAGE). Influence of purified AK autoAbs on cultured human keratinocytes was stud ied with MTT colorimetric determination and cell cycle analysis. RESULTS Purified antibodies were pure enough for electrophoretic analysis. Results from MTT colorimetric determin ation demonstrated that AK autoAbs have significant dose-dependent inhibitory effect on cultured keratinocytes. Correlation coefficient was -0.797. Cell cycle analysis showed th at after treatment with 0.01125 g*L-1 AK autoAbs for 72 h, the proportion of cells in G2/M + S phase decreased from 56.2% to 46.7%, and the proportion in G1/G0 phase correspondingly increased 43.8% to 53.3%. CONCLUSION Natural AK autoAbs, purified fro m normal human serum, have inhibitor y effect on the proliferation of human keratinocytes cultured in vitro.
- [1]J A, Girault,F S, Gorelick,P, Greengard.Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns.[J].1989,182(1).
- [2]C, Ackermann-Schopf,R, Ackermann,P I, Terasaki,etc.Natural and acquired epidermal autoantibodies in man.[J].1974,112(6).
- [3]Antonio Coutinho,Michel D Kazatchkine,Stratis Avrameas.Natural autoantibodies[J].1995,7(6).
- [4]K, Iwatsuki,J, Viac,A, Reano,etc.Comparative studies on naturally occurring antikeratin antibodies in human sera.[J].1986,87(2).
- [5]E P, Paluch,K J, Bloch.Antibodies to human epidermal cytoplasmic antigens: incidence, patterns, and titers.[J].1982,79(2).
- [6]S. Avrameas,G. Dighiero,P. Lymberi,etc.Studies on natural antibodiesand autoantibodies[J].1983,134(Part 4).
- [7]R, Gonzalez,J, Charlemagne,W, Mahana,etc.Specificity of natural serum antibodies present in phylogenetically distinct fish species.[J].1988,63(1).
- [8]Serre, Guy,Vincent, Christian,Viraben, Roland,etc.Natural IgM and IgG Autoantibodies to Epidermal Keratins in Normal Human Sera. I: ELISA-Titration, Immunofluorescence Study.[J].1987,88(1).
- [9]H, Hintner,T J, Lawley.Keratin intermediate filaments bear antigenic determinants for stratum corneum antibodies.[J].1984,82(5).
- [10]Zhi Jian Chen,Jim Wheeler,Abner Louis Notkins.Antigen-binding B cells and polyreactive antibodies[J].1995,25(2).
- [11]E A, Abel,J C, Bystryn.Epidermal cytoplasmic antibodies: incidence and type in normal persons and patients with melanoma.[J].1976,66(1).
- [12]胡大海,Margaret A.Hughes,陈璧,等.应用MTT法观察人皮肤培养细胞的形态学特征[J].1999,(11).
- [13]Y F, Liu,Y F, Liu,T S, Zhang,etc.Immunohistochemical study of anti-keratin autoantibodies in both normal and pathological state.[J].1993,106.
- [14]G, Grubauer,N, Romani,H, Kofler,etc.Apoptotic keratin bodies as autoantigen causing the production of IgM-anti-keratin intermediate filament autoantibodies.[J].1986,87(4).
- [15]Hintner H,Steinert P M,Lawley T J.Human upper epidermal cytoplasmic antibodies are directed against keratin intermediate filament proteins.[J].1983.
- [16]Y, Tomer,Y, Shoenfeld.The significance of natural autoantibodies.[J].1988,17(5).
- [17]S. Avrameas.Natural autoantibodies: from ‘horror autotoxicus’ to ‘gnothi seauton’[J].1991,12(5).
- [18]T K, Burnham.Two epidermal cytoplasmic immunofluorescent patterns detected by indirect immunofluorescence.[J].1974,62(2).
- [19]K. IWATSUKI,S. IMAIZUMI,H. HASHIZUME,etc.Production of antikeratin autoantibodies by hybrid spleen cells of naive mice[J].1990,123(6).
- [20]S V, Boyden.Natural antibodies and the immune response.[J].1966,5.
- [21]Gabriel Bikah,Jacqueline Carey,John R. Ciallella,etc.CD5-Mediated Negative Regulation of Antigen Receptor-Induced Growth Signals in B-1 B Cells[J].1996,274(5294).
- [22]S C, Liu,M, Karasek.Isolation and growth of adult human epidermal keratinocytes in cell culture.[J].1978,71(2).
- [23]Hintner, Helmut,Romani, Nikolaus,Stanzl, Ursula,etc.Phagocytosis of Keratin Filament Aggregates Following Opsonization With IgG-Anti-Keratin Filament Autoantibodies.[J].1987,88(2).
- [24]Lacroix-Desmazes S,Malanchere E,Kazatchkine MD,etc.Self-reactive antibodies (natural autoantibodies) in healthy individuals.[J].1998,216(1).
- [25]G.C. PRIESTLEY,R. LORD.Fibroblast-keratinocyte interactions in psoriasis: failure of psoriatic fibroblasts to stimulate keratinocyte proliferation in vitro[J].1990,123(4).
- [26].Research needs in 11 major areas in dermatology. I. Psoriasis.[J].1979,73.
- [27]K, Hayakawa,M, Asano,S A, Shinton,etc.Positive selection of natural autoreactive B cells.[J].1999,285(5424).