Abstract: AIM To investigate the effects of circadian rhythm an d repeated testi ng on event related potential (ERP) P300 component. METHODS Every subject (n=8) was tes-ted with a standard aural oddball paradigm to obtain ERP P300 . They w ere tested at 5 timepoints (7:00, 12:00, 16:00, 20:00, 0:00) per day in 5 days w ith an interval of at least 1 wk between 2 test days. In each test ERP was recorded. RESULTS Amplitude and latency of P300 changed with the time of the day. Th e amplitude of P300 was significantly higher in the morning than in the af ternoo n. It also habituated across test days from a mean of 13.0 μV on the 1st day t o a mean of 9.2 μV on the 5th day. Latency of P300 also habituated across test da ys; it decreased from a mean of 350.6 ms on the 2nd day to a mean of 343.5 ms on the 5th day. CONCLUSION Both circadian rhythm and repeated testing can influence amplitude and latency of ERP P300.