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In situ hybridization of gonadotropin releasing hormone receptor mRNA in rat pancreas
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2001.01.003
- Key Word:
- 促性腺素释放激素(GnRH);受体mRNA;原位杂交;胰脏;大鼠
Abstract： AIM To study whether gonadotropin releasing hormone receptor (GnRHR) mRNA exists in rat pancreas and its cellular localization. METHODS In situ hybridization with digoxigenin-labeled oligonucleotide probes was adopted. RESULTS Pancreatic endocrine islet cells, exocrine glandular cells and exocrine duct epithelial cells were all found to have GnRHR mRNA hybridization signals. The signals distributed in cytoplasm of all positive cells with negative nucli. CONCLUSION Both of the exocrine and endocrine cells of the pancrea can produce GnRH recepor. GnRH is also a kind of digestive hormone produced by pancrea and is responsible for modulating the exocrine and endocrine function.
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