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Noxious stimulation to stomach effects on neurons containing SP mRNA in premamillary region of rat hypothalamus
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FORTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 12
- DOI:
- 10.3321/j.issn:1000-2790.2000.12.001
- Key Word:
- SP mRNA;伤害性刺激;胃;乳头体前区;原位杂交;大鼠
Abstract： 目的观察正常和胃伤害性刺激大鼠下丘脑乳头体前区(PMH)内 P物质(SP) mRNA阳性神经元的分布和变化.方法福尔马林导入胃制成内脏伤害性刺激模型,用原位杂交组织化学法观察.结果对照组的PMH内SP mRNA阳性神经元的数量较少.中等强度的SP mRNA杂交信号主要见于乳头体内侧核的中间部和外侧部、乳头体外侧核和弓状核.给予胃伤害性刺激后,PMH内表达SP mRNA的神经元的数量明显增加,较强的杂交信号见于乳头体上交叉腹外侧部,乳头体内侧核内侧部、中间部和外侧部,结节乳头体核尾侧部,乳头体前核腹侧部和背侧部;中等强度的杂交信号见于弓状核、乳头体上核和乳头体外侧核背侧部;较弱的杂交信号见于下丘脑中介核、结节大细胞核,下丘脑背内侧核、下丘脑后区及背侧部、丘脑底核、穹窿背外侧部、下丘脑外侧区和乳头体被盖区吻侧部.结论大鼠下丘脑乳头体前区内有表达SP mRNA的神经元,其中的部分神经元可能参与了SP 介导的、对一般内脏感觉信息传递的调控.
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