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Angiostatin kringle (1-3) induces vascular endothelial cell apoptosis in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2000.09.004
- Key Word:
- angiostatin;内皮细胞;凋亡
Abstract： 目的探讨angiostatin kringle (1-3)[简称AK(1-3)]抑制血管内皮细胞增殖的作用机制.方法以加入重组人AK(1-3)蛋白(300 nmol.L-1)的含100 mL.L-1小牛血清DMEM培养基培养人脐静脉内皮细胞ECV304,72 h 后,采用透射电镜、流式细胞术细胞周期分析和核DNA琼脂糖凝胶(15 g.L-1)电泳检测重组AK(1-3)蛋白作用后血管内皮细胞的凋亡情况.结果实验组ECV304细胞检测到了典型的凋亡.透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状.对照组ECV304细胞表现正常.结论重组人AK(1-3)蛋白诱导了血管内皮细胞的凋亡,这是其抑制血管内皮细胞增殖的机制之一.
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