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Expression significance of extra-originated angiostatinK(1-3) gene in human gliomas
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2000.09.003
- Key Word:
- 血管抑素K(1-3);胶质瘤;表达;治疗
Abstract： 目的探讨外源性血管抑素K(1-3)基因[angiostatinK(1-3),AK(1-3)]在人脑胶质瘤中的表达及其作用.方法应用PCR法,使人Ig kappa链分泌信号肽序列S加装在AK(1-3)基因上-SAK(1-3);构建SAK(1-3)基因的真核表达载体pcDNA-SAK(1-3).经脂质体法将其转染入SHG44人脑胶质瘤细胞,行G418筛选获得转基因瘤细胞克隆,比较转基因和未转基因的瘤细胞生物学特性.以免疫荧光、免疫组化和内皮细胞抑制实验检测瘤细胞表达的AK(1-3)蛋白及其活性.应用裸鼠皮下致瘤性实验,结合SHG44瘤组织的各项检测,确定肿瘤表达AK(1-3)蛋白对人脑胶质瘤生长的影响.结果外源性AK(1-3)的表达不影响SHG44瘤细胞的生物学特性,它能在体外抑制内皮细胞生长;表达AK(1-3)的胶质瘤细胞体内致瘤性显著下降,抑瘤率为76.8%.结论肿瘤表达的AK(1-3)蛋白能抑制其自身生长,该机制是通过抑制胶质瘤的血管生成,进而导致肿瘤局部缺血坏死实现的.
- [1]吴景文,章翔,柴玉波,等.血管抑素K（1~3）基因片段的克隆与序列测定[J].2000,(1).
- [2]吴景文,章翔,高大宽,等.Angiostatin K（1-3）基因的原核表达和活性鉴定[J].2000,(2).
- [3]B K, Sim,M S, O'Reilly,H, Liang,etc.A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer.[J].1997,57.
- [4]T, Boehm,J, Folkman,T, Browder,etc.Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance.[J].1997,390(6658).
- [5]Flynn E,Cao Y,Marshall B,etc.Expression of angiostatin cDNA in a murine fibrosarcoma suppresses primary tumor growth and produces long-term dormancy of metastases.[J].1998,101(5).
- [6]Griscelli F,Opolon P,Li H,etc.Angiostatin gene transfer: inhibition of tumor growth in vivo by blockage of endothelial cell proliferation associated with a mitosis arrest.[J].1998,95(11).