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Effects of psoralen and 8 methoxypsoralen on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 8
- DOI:
- 10.3321/j.issn:1000-2790.2000.08.001
- Key Word:
- 涎腺;粘液表皮样癌;肿瘤转移;补骨脂素;8-甲氧基补骨脂素
Abstract： 目的研究补骨脂素(PSO)和8-甲氧基补骨脂素(8-MOP)对涎腺粘液表皮样癌高转移细胞克隆Mc3恶性表型的影响.方法用MTT法、细胞记数法、软琼脂克隆形成法、流式细胞术、癌细胞静脉注射法(每只鼠106个细胞)等研究PSO和8-MOP对Mc3细胞增殖以及转移力的抑制作用.结果PSO和8-MOP对Mc3细胞具有浓度依赖性生长抑制作用,IC30(mg.L-1)分别为32.4和25.1,IC50(mg.L-1)分别为44.7和35.5.IC30浓度的药物作用于Mc3细胞5d后对照组、PSO组和8-MOP组细胞的倍增时间(h)分别为20.8,23.3和57.8;S期细胞所占比例(%)为24.5,17.8和5.8;p53蛋白阳性表达率(%)为24.0,99.8和99.0;nm23-H1蛋白表达率(%)为99.9,99.5和99.1;克隆形成率(%)为23.5,16.5和0;在裸鼠肺表面的转移结节数为139±61,114±68和36±32.结论IC30的PSO和8-MOP可抑制Mc3细胞的转移力,8-MOP作用更显著.
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