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SPASE for rapid detection of serum TIMP-1 in patients with liver cirrhosis
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2000.07.005
- Key Word:
- 固相致敏红细胞吸附技术;肝硬化;金属蛋白酶组织抑制因子-1;免疫检测
Abstract： 目的建立一种改良固相致敏红细胞吸附技术(SPASE)用于快速检测肝硬化患者血清中TIMP-1. 方法用TIMP-1单克隆抗体包被微量血凝板,加热冲洗后加入待检血清标本,最后加入单克隆抗体致敏红细胞. 应用致敏红细胞作为指示系统,观察红细胞吸附状况来判定结果. 结果 SPA SE法全过程仅需要2.5～3 h,408例肝病患者血清检测TIMP-1结果为急性肝炎组检出阳性率16.4%;慢性肝炎组阳性率为33.3%;肝硬化组阳性率为73.6%. 结论血清中TIMP-1的变化可作为肝纤维化较为有用的诊断指标. SPASE法具有较高的敏感性、特异性和快速性,试剂用量少,且不需复杂的仪器设备,实验程序又简单,很适合医院及基层医疗单位应用.
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