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Tissue inhibitors of metalloproteinase-1 and -2 (TIMP-1 and TIMP-2) related antigens expression in liver tissues of patients with liver cirrhosis
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2000.07.004
- Key Word:
- 肝硬化;金属蛋白酶组织抑制因子;细胞外基质;金属蛋白酶;抗原;免疫组织化学
Abstract： 目的了解TIMP-1和TIMP-2在肝硬化患者肝组织中的表达状态,探讨 TIMP-1和TIMP-2在肝硬化发病机制中的作用. 方法以抗TIMP-1和TIM P-2单克隆抗体(McAb)为试剂,采用免疫组织化学法检测肝硬化患者肝组织中TIMP-1,TIMP-2相关抗原的表达. 结果 30例肝硬化患者肝组织中TIMP-1和TIMP-2相关抗原表达的阳性率为100%,,TIMP-1阳性的肝组织中TIMP- 2亦为阳性,且TIMP-1强度高于TIMP-2,而正常肝组织无1例表达阳性. TIMP-1和TIMP-2 抗原表达阳性信号主要位于肝细胞胞质中,未见细胞核表达. 结论肝硬化患者 肝组织中的肝细胞中存在TIMP-1和TIMP-2相关抗原表达,TIMP-1和TIMP-2与肝病的进展相关,它通过抑制MMPs 的活性,使得ECM降解减少而导致肝纤维化,以至肝硬化.
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