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The effect of telomerase antisense RNA on HL-60
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2000.04.040
- Key Word:
- 端粒酶;反义核酸;白血病;基因转染;细胞凋亡
Abstract： 目的探讨抗端粒酶在急性白血病治疗中的意义. 方法用脂质体介导法将pBBS212-hT R(反义端粒酶RNA真核表达载体)导入人髓性白血病细胞系HL-60中,采用TRAP法测定端粒酶活性,细胞生长动力学检测,电镜,DNA片段分析等方法观察其对HL-60细胞的端粒酶活性、细胞增殖及细胞凋亡影响. 结果端粒酶反义RNA能显著抑制HL-60细胞的端粒酶活性,抑制H L-60细胞的增殖并促进其凋亡. 结论以端粒酶反义RNA抑制端粒酶活性是治疗白血病的潜在途径.
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