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Detection of replication-competent retrovirus in retroviral vector mediated gene therapy
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 12
- DOI:
- 10.3321/j.issn:1000-2790.1999.12.004
- Key Word:
- 可复制逆转录病毒;基因治疗;包装细胞
Abstract： 目的:探讨逆转录病毒载体介导的基因治疗中,可复制逆转录病毒(RCR)的检测方法,为其在应用中的安全性奠定基础.方法:构建含抗HIV-1整合酶单链抗体基因的逆转录病毒表达载体(pSLXCMV/IN-sFv),应用3T3放大实验继S+/L-试验对其包装细胞(PA317/IN-sFv)上清中RCR进行了检测,并以野生型4070A病毒为阳性对照.结果:含IN-sFv的包装细胞上清中RCR呈阴性,不同稀释度的阳性对照中,可见明确的灶状细胞并与已知病毒滴度相符.结论:该结果进一步证实了上述RCR检测系统的可靠性,同时也是我们以sFv为目的基因进行逆转录病毒载体介导基因治疗临床试验的可靠指标.
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