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Effect of ricin modified with and without SPDP on GSH levels in mice liver
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.1999.10.004
- Key Word:
- 蓖麻毒素;化学修饰;3-(2-吡啶二巯基)丙酸N-羟基琥珀酰亚胺酯;还原型谷胱甘肽;小鼠
Abstract： 目的: 观察蓖麻毒素(RT)修饰前后对小鼠肝脏毒性的影响. 方法: RT用3-(2-吡啶二巯基)丙酸N-羟基琥珀酰亚胺酯(SPDP)一种异型双功能交联剂,进行化学修饰;生成RT的SPDP衍生物(PDP-R),测定两者在不同剂量和时间对小鼠肝脏还原型谷胱甘肽(GSH)含量的影响,以比较两者的毒性差别. 结果: 随着中毒剂量的增加和时间的延长,两者均可使小鼠肝脏GSH含量下降(P<0.01),但RT组下降程度较PDP-R组更加明显(P<0.01),表明PDP-R对小鼠毒性弱于RT. 结论: 用SPDP修饰RT可降低其对小鼠的毒性,这对改进RT抗癌作用可能是一种有意义的尝试.
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