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Preparation of native angiostatin from human plasma and its antiangiogenesis effects
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.1999.10.002
- Key Word:
- 血管生成;抑制物;蛋白纯化
Abstract： 目的: 从血浆中纯化制备有特异抑制血管生成活性的天然angiostatin蛋白,为基础研究和应用研究打下基础. 方法: 制备L-lysine偶联的Sepharose 4B亲和层析柱,从血浆中纯化纤溶酶原,用弹性蛋白酶在体外进行有限消化,再以L-lysine Sepharose 4B亲和层析纯化获得天然angiostatin片段,通过体外血管内皮细胞生长抑制分析和体内鸡胚尿囊膜血管生成抑制实验测定纯化蛋白的活性. 结果: 以L-lysine Sepharose 4B从血浆中纯化得到单一成分的纤溶酶原蛋白,弹性蛋白酶有限消化后进一步的亲和层析纯化得到angiostatin蛋白;该蛋白体外可抑制牛主动脉内皮细胞的生长,体内可抑制鸡胚尿囊膜血管生成. 结论: 该实验方法可纯化得到有活性的天然angiostatin蛋白.
- [1]Zhanggui Wu,Michael S. O'Reilly,Judah Folkman,etc.Suppression of Tumor Growth with Recombinant Murine Angiostatin[J].1997,236(3).
- [2]J, Folkman,C C, Haudenschild,B R, Zetter.Long-term culture of capillary endothelial cells.[J].1979,76(10).
- [3]P C, Brooks,A M, Montgomery,M, Rosenfeld,etc.Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels.[J].1994,79.
- [4]Marti D,Schaller J,Davidson D,etc.Kringle domains of human angiostatin. Characterization of the anti-proliferative activity on endothelial cells.[J].1996,271(46).
- [5]B K, Sim,M S, O'Reilly,H, Liang,etc.A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer.[J].1997,57.