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GDNF cloning and its expression in eukaryotic cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.1999.09.001
- Key Word:
- 胶质细胞源性神经营养因子;真核细胞;DNA;重组;限制酶图谱法;转染;逆转录聚合酶链反应
Abstract： 目的:构建pcDNA3/GDNF真核表达质粒并了解其在真核细胞内的表达.方法:将GDNF逆转录聚合酶链式反应产物克隆至pcDNA3真核表达载体上,经酶切鉴定及测序分析并以脂质体介导法转染真核细胞,了解其在细胞内的表达及其表达蛋白的生物学活性.结果:酶切鉴定及测序分析表明重组pcDNA3/GDNF表达质粒克隆成功,转染实验表明重组质粒能在真核动物细胞中表达出具有活性的GDNF蛋白.结论:以脂质体介导pcDNA3/GDNF质粒转染真核细胞有可能用于帕金森氏症的基因治疗.
- [1]U J, Kang.Potential of gene therapy for Parkinson's disease: neurobiologic issues and new developments in gene transfer methodologies.[J].1998,13 Suppl 1.
- [2]Michael Boshart,Frank Weber,Gerhard Jahn,etc.A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus[J].1985,41(2).
- [3]Andrew McQuillin,Karl Murray,Christopher Etheridge,etc.Optimization of liposome mediated transfection of a neuronal cell line[J].1997,8(6).
- [4]P A, Lapchak,D M, Gash,F, Collins,etc.Pharmacological activities of glial cell line-derived neurotrophic factor (GDNF): preclinical development and application to the treatment of Parkinson's disease.[J].1997,145.
- [5]L F, Lin,D H, Doherty,J D, Lile,etc.GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.[J].1993,260(5111).
- [6]Suter Crazzolara C,Unsicker K.GDNF mRNA levels are induced by FGF-2 in rat C6 glioblastoma cells.[J].1996,41(1).
- [7]Z, Hannas-Djebbara,M, Didier-Bazs,S, Sacchettoni,etc.Transgene expression of plasmid DNAs directed by viral or neural promoters in the rat brain.[J].1997,46.
- [8]D L, Choi-Lundberg,Q, Lin,Y N, Chang,etc.Dopaminergic neurons protected from degeneration by GDNF gene therapy.[J].1997,275(5301).