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Construction of pEGFP-HPV16E6/E7 expression vector and detection of transient expression in transfected keratinocytes

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
8
DOI:
10.3321/j.issn:1000-2790.1999.08.005
Key Word:
人乳头瘤病毒;表达载体;构建;基因转染

Abstract: 目的: 构建pEGFP-HPV16E6/E7表达载体,观察其在角朊细胞中的瞬时表达. 方法: 基因重组技术,基因转染技术. 结果: 酶切鉴定证实HPV16E6/E7片段已克隆到pEGFP-N3的EcoR I和BamH I位点之间,在转染角朊细胞中观察到绿色荧光蛋白的表达. 结论: pEGFP-HPV16E6/E7表达载体便于观察, 转染细胞中HPV16E6/E7-GFP融合蛋白的表达情况及蛋白定位,适用于对HPV16E6/E7分子生物学特性及致瘤机理的研究.

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