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Study on downregulation of c-myc and PCNA genes expression by antisense oligonucleotides in vascular smooth muscle cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 8
- DOI:
- 10.3321/j.issn:1000-2790.1999.08.002
- Key Word:
- 反义寡核苷酸;血管平滑肌;基因表达;原癌基因
Abstract： 目的: 利用反义寡核苷酸阻遏血管平滑肌细胞(VSMC)c-myc, PCNA基因表达,为移植血管再狭窄的基因治疗提供理论依据. 方法: 人工合成正义、反义及错配反义c-myc, PCNA基因片断,并转入体外培养的VSMC中,运用逆转录聚合酶链式反应(RT-PCR)测定细胞中相应基因表达的情况. 结果: 反义寡核苷酸能有效抑制VSMC中c-myc, PCNA基因的表达,而正义和错配反义寡核苷酸没有此效应. 结论: 反义寡核苷酸能阻遏VSMC中相应基因的表达.
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