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Preparation and experimental study of allogeneic acellular dermal matrix for composite skin grafting
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.1999.05.005
- Key Word:
- 脱细胞真皮基质;复合皮;皮肤移植
Abstract： 目的:寻找适合烧伤创面移植的异体脱细胞真皮基质(allo-ADM).方法:将薄中厚小猪皮采用胰蛋白酶等消化,制成含基底膜的ADM,再作组织学等检测和猪复合移植实验.结果:①allo-ADM具有无细胞、无细菌、低毒性等特点.②戊二醛不同交联时间组的ADM,与自体薄中厚皮片重叠移植于猪全层皮肤切除的创面上,皮片成活率为100%,早期观察无排斥反应,外观平整;5wk时组织学结构完整,炎性反应轻,随交联时间延长,allo-ADM内浸润生长的成纤维细胞、血管内皮细胞和炎性细胞逐渐减少,而未经交联的allo-ADM组织内炎性反应重,组织降解迅速.结论:发挥真皮支架作用的allo-ADM,宜采用低浓度戊二醛等作短时间的化学交联处理.
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