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Regulation of substance P in adhesion of eosinophil to endothelial cell
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.1999.04.014
- Key Word:
- 嗜酸性粒细胞;P物质;粘附
Abstract： 目的:探讨P物质(SP)对嗜酸性粒细胞(Eos)粘附功能的调节作用及机制.方法:用收集51Cr标记的白细胞分析法观察人Eos对培养的人脐带静脉血管内皮细胞(HUVEC)的粘附作用.在实验中以不同浓度SP和SP+血小板激活因子(PAF)分别预处理Eos,观察其对经IL-1β活化的HUVEC粘附性变化.以GR71251(NK-1受体阻断剂)和抗CD11b作为干预因素,观察它们对SP+PAF作用的影响.此外,我们还以SP预刺激HUVEC,观察其对经SP+PAF活化人Eos的粘附性变化.结果:单纯SP不能增加Eos对经IL-1β活化的HUVEC的粘附性,却能显著增加PAF的促粘效应,这一作用呈剂量依赖性并且被GR71251和抗CD11b所阻断,阻断率分别为29.2%和59.2%.SP预处理HUVEC后,HUVEC对SP+PAF活化的Eos的粘附性增强,也呈剂量依赖性,GR71251对此有显著的抑制作用.结论:SP能促进PAF对人Eos的粘附性,NK-1受体和CD11b可能参与这一过程;SP还能激活HUVEC,使其对Eos的粘附作用增强.
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