Effect of perinatal malnutrition on learning and memory function of newborn rats
- Author:
- No author available
- Journal Title:
- Acta Academiae Medicinae Militaris Tertiae
- Issue:
- 19
- DOI:
- No doi available
- Key Word:
- 围生期营养不良;学习;记忆;DNA损伤;DNA修复;大鼠
Abstract： 目的 研究围生期营养不良对仔鼠学习记忆的影响,探讨其可能的机制.方法 怀孕14 d的SD大鼠分为实验组(半量饮食组)与对照组(正常饮食组),至哺乳期结束(仔鼠出生21 d)后实验组和对照组的仔鼠均正常饮食.测量仔鼠出生时和成年时的体质量.采用Morris水迷宫测试仔鼠的学习、记忆功能.免疫组化检测DNA氧化损伤(8-OhdG)和Western blot检测DNA氧化损伤修复(OGG1)指标.结果 实验组仔鼠出生和成年后体质量均小于对照组[(4.66±0.28) g vs (6.59±0.16)g,(187.66 ±22.67)g vs(279.50±18.73)g,P＜0.01]；Morris水迷宫实验中,空间探索实验实验组较对照组潜伏时间长[(37.21 ±4.93) svs (16.41 ±5.21)s,P＜0.05],穿越平台次数较少[(6.57 ±1.22)vs(13.12 ±2.34),P＜0.05],跨越目标象限时间占整个游泳的时间百分率低[(32.75±4.58)％vs (60.31±6.74)％,P＜0.01],Morris水迷宫实验表明实验组学习记忆功能均比对照组下降；8-OHdG的表达在幼年期(22 d)和成年期(90 d)实验组均比较高,与对照组相比具有统计学意义[(18.01±0.05)vs(5.77 ±0.03),(21.15±0.10)vs(10.81 ±0.08),P＜0.05]；OGG1的表达在幼年期(22 d)和成年期(90 d)实验组均比较低,与对照组相比差异具有统计学意义[(0.32±0.08)vs(0.81±0.06),(1.57 ±0.10) vs (2.78 ±0.53),P＜0.05].结论 围生期营养不良对仔鼠成年期的学习记忆功能产生影响,其机制可能与DNA氧化损伤、修复调节的障碍有关.
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