IL-18 increases superoxide anion production in neutrophils of rats during resuscitation following burn shock
- Author:
- No author available
- Journal Title:
- Acta Academiae Medicinae Militaris Tertiae
- Issue:
- 19
- DOI:
- No doi available
- Key Word:
- 白细胞介素18;烧伤;休克;延迟复苏;超氧化物类;羟自由基
Abstract： 目的 探讨在烧伤休克复苏大鼠模型中,IL-18与中性粒细胞释放氧自由基——超氧阴离子和羟自由基的关系以及相应的作用.方法 建立烧伤休克延迟复苏模型大鼠,分别检测假烧伤组以及延迟复苏大鼠在模型建立后6、12、24、48 h体内白介素-18(interleukin 18,IL-18)以及中性粒细胞的含量.腹腔注射IL-18(20μg/kg)以及抗大鼠IL-18抗体(anti-rat-IL-18 antibody,80 μg/kg)后,检测模型大鼠中性粒细胞内超氧自由基以及羟自由基的含量.将新鲜分离的中性粒细胞体外培养,分别加入IL-18(0.5 μg/ml)以及anti-rat-IL-18抗体(0.5μg/ml),共培养24 h后,检测中性粒细胞内超氧自由基以及羟自由基的含量.结果 大鼠烫伤后6、12、24、48 h外周血IL-18的含量[(51.46±11.36)、(64.86±12.05)、(78.64±15.64)、(69.44±12.28) pg/ml]以及中性粒细胞的数量[(38.46±13.57)×105、(128.45 ±45.78)×105、(245.25 ±84.15)×105、( 179.56 ±49.27)×105/ml]均显著提高,且变化趋势相同(P＜0.01).腹腔注射IL-18大鼠中性粒细胞内超氧阴离子以及8-羟基脱氧鸟苷(8-OHdG)的含量均显著高于假烧伤组[(0.45 -±0.03)vs(0.15 ±0.02),(605±145)vs( 152 ±21) pg/ml,P＜0.01],而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子以及8-OHdG的含量均显著低于烧伤大鼠延迟复苏组[ (0.19 ±0.03)vs(0.32 ±0.04),(198 ±45) vs (524±124) pg/ml,P＜0.01].离体条件下,加入IL-18的中性粒细胞在体外超氧阴离子以及8-OHdG的含量均显著高于对照组[(0.38±0.02)vs(0.13±0.02),(505 ±99)vs(92 ±21) pg/ml,P＜0.01],而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子以及8-OHdG的含量均显著低于烧伤组大鼠[(0.16±0.03) vs (0.29±0.03),(95 ±45)vs(324 ±68) pg/ml,P＜0.01].结论 在烧伤休克延迟复苏大鼠中,IL-18能够有效地与大鼠体内中性粒细胞结合,促进烧伤休克延迟复苏大鼠模型中的氧自由基的生成,进一步造成组织的损伤.
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