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Effect of protease inhibitor MG132 on cancer cachexia
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- Author:
- No author available
- Journal Title:
- Acta Academiae Medicinae Militaris Tertiae
- Issue:
- 19
- DOI:
- No doi available
- Key Word:
- 蛋白酶体抑制剂;MG132;肿瘤恶病质
Abstract： 目的 研究不同剂量蛋白酶体抑制剂MG132对肿瘤恶病质的预防和治疗作用.方法 采用小鼠结肠腺癌C26细胞接种BALB/c小鼠诱导的肿瘤恶病质模型.分为对照组(HC),肿瘤恶病质组(CC),MG132预防的低浓度组(PL),中浓度组(PM)和高浓度组(PH),MG132治疗的低浓度组(TL)、中浓度组(TM)和高浓度组(TH).每天监测小鼠体质量、饮食量和肿瘤体积,预防组和治疗组分别于接种后第5、12天开始每天腹腔注射MG132(0.01、0.1、0.5 mg/kg).第19天各组处死8只小鼠,称量肿瘤、左侧腓肠肌和附睾脂肪组织质量,检测血清葡萄糖( Glu)、甘油三酯(TG)、总蛋白(TP)和白蛋白(ALB)水平.CC、PM和TM组剩余8只小鼠继续原治疗至其死亡,观察生存时间.结果 与CC组相比,MG132预防和治疗组小鼠自发活动量、去瘤体质量、腓肠肌纤维横切面积、腓肠肌和附睾脂肪质量增加,Glu和ALB水平升高,TG水平降低,生存时间延长,均以PM和TM组效果最好,且两组间以上指标差异均有统计学意义(P ＜0.05)；PH组最显著地抑制了肿瘤生长(P＜0.05).结论 MG132的中浓度剂量能较好地改善恶病质,延长生存时间,且预防效果更明显.
- [1]周伟,江志伟,姜军,等.一种癌性恶病质动物模型的建立[J].2004,(4).
- [2]Yuedong Ma,Baolin Chen,Dan Liu,etc.MG132 treatment attenuates cardiac remodeling and dysfunction following aortic banding in rats via the NF-κB/TGFβ1 pathway[J].2011,81(10).
- [3]Prado CM,Lieffers JR,McCargar LJ,etc.Prevalence and clinical implications of sarcopenic obesity in patients with solid tumours of the respiratory and gastrointestinal tracts: a population-based study.[J].2008,9(7).
- [4]José Cláudio Fonseca Moreira,Ana Maria Oliveira Battastini,Elizandra Braganhol,etc.Proteasome inhibitor MG132 induces selective apoptosis in glioblastoma cells through inhibition of PI3K/Akt and NFkappaB pathways, mitochondrial dysfunction, and activation of p38-JNK1/2 signaling.[J].2012,30(6).
- [5]Mittal A,Bhatnagar S,Kumar A,etc.The TWEAK-Fn14 system is a critical regulator of denervation-induced skeletal muscle atrophy in mice.[J].2010,188(6).
- [6]Fearon K,Strasser F,Anker SD,etc.Definition and classification of cancer cachexia: an international consensus.[J].2011,12(5).
- [7]Zhang L,Hu JJ,Gong F.MG132 inhibition of proteasome blocks apoptosis induced by severe DNA damage.[J].2011,10(20).
- [8]Tisdale MJ.Mechanisms of cancer cachexia.[J].2009,89(2).
- [9]Annabelle Z Caron,Sonia Haroun,Élisabeth Leblanc,etc.The proteasome inhibitor MG132 reduces immobilization-induced skeletal muscle atrophy in mice[J].2011,12(1).
- [10]岳彩霞,马骥,周海俊,等.蛋白酶体抑制剂MG132对RhoA诱导的肿瘤血管生成的抑制作用的研究[J].2011,(4).
- [11]Shontelle, Dodson,Vickie E, Baracos,Aminah, Jatoi,etc.Muscle wasting in cancer cachexia: clinical implications, diagnosis, and emerging treatment strategies.[J].2011,62.
- [12]Melstrom LG,Melstrom-KA Jr,Ding XZ,etc.Mechanisms of skeletal muscle degradation and its therapy in cancer cachexia.[J].2007,22(7).
- [13]Francaux M,Li An G,Raymackers JM,etc.Prevention of muscle disuse atrophy by MG132 proteasome inhibitor.[J].2011,43(5).
- [14]von Haehling Stephan,Anker Stefan D..Cachexia as a major underestimated and unmet medical need: facts and numbers[J].2010,1(1).