You Position: Home > Paper
Crystal structure of Vibrio cholerae HutX protein
( views:165, downloads:0 )
- Author:
- No author available
- Journal Title:
- Academic Journal of Second Military Medical University
- Issue:
- 9
- DOI:
- 10.3724/SP.J.1008.2012.00969
- Key Word:
- 霍乱弧菌;HutX蛋白;X线结晶学;血红素利用
Abstract： 目的 获取纯化的霍乱弧菌HutX蛋白晶体和衍射数据.方法 通过基因克隆和蛋白表达得到HutX蛋白,通过镍柱亲和层析、Source Q阴离子交换柱层析和Superdex 200分子筛柱层析等纯化所得蛋白,并用蛋白质印迹法进行验证.将所获蛋白进行结晶条件筛选及悬滴优化,X线衍射分析所得晶体的结构.结果 蛋白质印迹分析间接证明所得到的蛋白为HutX蛋白,并得到HutX蛋白的晶体及衍射数据.结论 为进一步研究HutX的蛋白结构及其在霍乱弧菌亚铁血红素利用机制中发挥的作用提供了参考.
- [1]G T, DeTitta,J R, Luft.Rate of water equilibration in vapor-diffusion crystallization: dependence on the residual pressure of air in the vapor space.[J].1995,51.
- [2]Ruby, E.G.,Dunn, A.K.,Wang, Y.,etc.The haem-uptake gene cluster in Vibrio fischeri is regulated by Fur and contributes to symbiotic colonization[J].2011,13(11).
- [3]Payne SM,Mey AR,Wyckoff EE.Iron acquisition in Vibrio cholerae[J].2007,20(3).
- [4]Beyhan S,Sandkvist M,Bagdasarian M,etc.Cell envelope perturbation induces oxidative stress and changes in iron homeostasis in Vibrio cholerae[J].2009,191(17).
- [5]O'Malley, S.M.,Mouton, S.L..Comparison of the heme iron utilization systems of pathogenic vibrios.[J].1999,181(11).
- [6]Lang J,Pal GP,Couture M,etc.Structure and heme binding properties of Escherichia coli O157:H7 ChuX.[J].2009,18(4).