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Establishment of a fluorescence-labeled human ovarian cancer cell line
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- Author:
- No author available
- Journal Title:
- Academic Journal of Second Military Medical University
- Issue:
- 9
- DOI:
- 10.3724/SP.J.1008.2012.00950
- Key Word:
- 卵巢肿瘤;HO8910PM细胞株;绿色荧光蛋白质类;转染
Abstract： 目的 建立稳定高表达增强型绿色荧光蛋白(EGFP)的人卵巢癌细胞株.方法 采用基因转染的方法,将EGFP基因导入人卵巢癌细胞HO8910PM中,通过G418筛选、亚克隆扩增获得稳定表达绿色荧光蛋白的EGFP-HO8910PM细胞株,并用流式细胞术检测所获细胞株的EGFP表达率,通过细胞生长曲线、黏附实验、侵袭迁移实验比较EGFP-HO8910PM细胞和HO8910PM细胞的生物学行为.结果 筛选出的EGFP-HO8910PM细胞经流式细胞仪检测EGFP阳性表达率达99％以上,EGFP-HO8910PM细胞和HO8910PM细胞生长、黏附及侵袭迁移能力无统计学差异.结论 成功建立了稳定表达EGFP且保持母株细胞特性的人卵巢癌细胞株EGFP-HO8910PM,为人卵巢癌整体活体应用中可视化研究打下基础.
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