Differential expression proteins detected by mass spectrometry in patients with T cell non-Hondgkin's lymphoma and their clinical value

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Author:
LIU Zong-jin(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
ZHANG Qiao-hua()
HOU Shu-ling()
GUO Su-tang()
YANG Rui-hong()
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 21, Issue 08, 2012
DOI:
10.3760/cma.j.issn.1009-9921.2012.08.006
Key Word:
Spectrometry, mass, matrix-assisted laser desorption-ionization; T-lymphocytes;Lymphoma, non-Hodgkin's

Abstract: Objective To find differential expression proteins in patients with T cell non-Hodgkin’ s lymphoma (T-NHL) by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique and study their related clinical application value and prospect.Methods Serum protein of 36 T-NHL patients and 30 DLBCL patients were detected by the SELD1-TOF-MS technique and weak cation exchange (wcx-2) chip.Lactate dehydrogenase (LDH) was detected by biochemistry method.Beta2-microglobulin (β2-MG) was detected by enzyme-linked immunesorbent assay (ELISA).The significant different protein spectrometry were analyzed between DLBCL patients and T-NHL patients.The correlation analysis with protein spectrometry,disease staging,LDH and β2-MG were analyzed with Spearman.Results Nine potential candidate proteins,including the peak intensity of M/Z 1142.67,1451.43,1472.49,1512.03,3194.22,3267.41,3933.86,4593.12 and 9182.24,were identified in T-NHL patients.The 9 protein markers had no contact with disease staging of T-NHL (P > 0.05).The protein markers of 4593.12 and 9182.24 were high level in T-NHL patients.LDH in these two protein markers’ positive group [(290.82±29.95) U/L,(283.94±100.94) U/L] was higher than that in negative group [(169.22±55.42) U/L,(169.50±59.25) U/L](t =-3.199,P =0.004; t =-2.378,P =0.026),and LDH was positive correlation with these two protein spectrometry (r =0.265,r =0.178,P < 0.01).There was no statistically significant difference ofβ2-MG between these two protein markers’ positive group and negative group (P > 0.05).The other 7 protein markers were low level in T-NHL patients,and there was no statistically significant difference of LDH and β2-MG in these 7 protein markers (P > 0.05).Conclusion The protein marker of 4593.12 and 9182.24 may be the specific serological markers to identify T-NHL.The combination of these two protein markers and LDH may assess the tumor load,and provide guiding value for clinical treatment.

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