Differential expression proteins detected by mass spectrometry in patients with T cell non-Hondgkin's lymphoma and their clinical value

( views:111, downloads:0 )
LIU Zong-jin(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
ZHANG Qiao-hua()
HOU Shu-ling()
GUO Su-tang()
YANG Rui-hong()
Journal Title:
Journal of Leukemia & Lymphoma
Volume 21, Issue 08, 2012
Key Word:
Spectrometry, mass, matrix-assisted laser desorption-ionization; T-lymphocytes;Lymphoma, non-Hodgkin's

Abstract: Objective To find differential expression proteins in patients with T cell non-Hodgkin’ s lymphoma (T-NHL) by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique and study their related clinical application value and prospect.Methods Serum protein of 36 T-NHL patients and 30 DLBCL patients were detected by the SELD1-TOF-MS technique and weak cation exchange (wcx-2) chip.Lactate dehydrogenase (LDH) was detected by biochemistry method.Beta2-microglobulin (β2-MG) was detected by enzyme-linked immunesorbent assay (ELISA).The significant different protein spectrometry were analyzed between DLBCL patients and T-NHL patients.The correlation analysis with protein spectrometry,disease staging,LDH and β2-MG were analyzed with Spearman.Results Nine potential candidate proteins,including the peak intensity of M/Z 1142.67,1451.43,1472.49,1512.03,3194.22,3267.41,3933.86,4593.12 and 9182.24,were identified in T-NHL patients.The 9 protein markers had no contact with disease staging of T-NHL (P > 0.05).The protein markers of 4593.12 and 9182.24 were high level in T-NHL patients.LDH in these two protein markers’ positive group [(290.82±29.95) U/L,(283.94±100.94) U/L] was higher than that in negative group [(169.22±55.42) U/L,(169.50±59.25) U/L](t =-3.199,P =0.004; t =-2.378,P =0.026),and LDH was positive correlation with these two protein spectrometry (r =0.265,r =0.178,P < 0.01).There was no statistically significant difference ofβ2-MG between these two protein markers’ positive group and negative group (P > 0.05).The other 7 protein markers were low level in T-NHL patients,and there was no statistically significant difference of LDH and β2-MG in these 7 protein markers (P > 0.05).Conclusion The protein marker of 4593.12 and 9182.24 may be the specific serological markers to identify T-NHL.The combination of these two protein markers and LDH may assess the tumor load,and provide guiding value for clinical treatment.

  • [1]The Non-Hodgkin’s Lymphoma Classification Project.A clinical evaluation of the international lymphoma study group classification of non-Hodgkin’s lymphoma.Blood,1997,89:3909-3918.
  • [2]Evans LS,Hancock BW.Non-Hodgkin’s lymphoma.Lancet,2003,362:139.
  • [3]克晓燕.淋巴瘤诊疗手册.北京:人民卫生出版社,2010:3-6,25.
  • [4]Liu C.The application of SELDI-TOF-MS in clinical diagnosis of cancers.J Biomed Biotech,2011,3:1-4.
  • [5]裴毅,郭素堂,王清馨,等.一组新发现的肿瘤功能性蛋白质的指纹图谱.肿瘤研究与临床,2005,17:153.
  • [6]王军,张巧花,郭素堂,等.蛋白质芯片技术在B细胞淋巴瘤病理分型及预后方面的应用.白血病·淋巴瘤,2009,18:407-409.
  • [7]张健,陶朝晖,景莉,等.表面增强激光解吸-电离-飞行时间质谱技术检测不同来源淋巴瘤细胞株及健康人外周淋巴细胞的差异蛋白质.白血病·淋巴瘤,2010,19:486-488.
  • [8]Janssen-Heijnen ML,Van Spronsen D J,Lemmens VE,et al.A population-based study of severity of comorbidity among patients with non-Hodgkin’s lymphoma:prognostic impact independent of International Prognostic Index.Br J Hematol,2005,129:597.
  • [9]庞丽萍,魏颖慧,张文丽,等.血清CA125、LDH、β2-MG在非霍奇金淋巴瘤诊断治疗中的意义.白血病·淋巴瘤,2006,15:113-114.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn