Relationship between methylation status in promoter region of Runx3 gene and children malignant lymphoma

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Author:
QU Fan(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
MENG Jian-hui(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
YANG Ming-juan(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
DIAO Yu-qiao(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
ZHU Xiu-li(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
LI Mei(Department of Pedeatrics, the Fourth Hospital of Hebei Medical Universty, Shijiazhuang 050011, China)
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 21, Issue 08, 2012
DOI:
10.3760/cma.j.issn.1009-9921.2012.08.005
Key Word:
Gene, Runx3; Methylation; Lymphoma; Child

Abstract: Objective To study methylation status of the Runx3 gene in occurrence and development of children malignant lymphoma.Methods The bone marrow specimens of 48 children diagnosed as malignant lymphoma were included into experimental group.20 children with non-malignancy were included into control group.Methylation-specific polymerase chain reaction (MS-PCR) was used to detect methylation status of Runx3 gene in bone marrow cells.The reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of Runx3 gene.Results MS-PCR assay results showed that 31 cases expressed Runx3 gene methylation in experimental group,the positive rate was 64.6 % (31/48),but no one was detected in control group (20 cases),the difference between two groups was significant (x2 =15.7,P <0.05).Dynamic observation of 42 cases in experimental group showed the declining of Runx3 methylation positive rate.RT-PCR assay results showed that all 20 cases in the control group expressed Runx3 gene mRNA,all cases with methylation status of the Runx3 gene in the experimental group didn’ t expressed Runx3 gene mRNA.In experimental group,9 cases of clinical remission expressed Runx3 gene mRNA,and 1 case of relapsed didn’ t expressed Runx3 gene mRNA.Conclusion Runx3 gene shows a high methylation status in bone marrow cells of malignant lymphoma children,so that blocked the expression of Runx3 gene,which is closely related to the occurrence and development of children malignant lymphoma.

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