Study on detection of common fusion genes in acute lymphoblastic leukemia

( views:244, downloads:0 )
Author:
ZHAO Jin(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
SU Li-ping(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
GUAN Tao(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
YAN Xiao-jun(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
FENG Kai-chao()
WANG Jun(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
MA Li(Department of Hematology, Shanxi Cancer Hospital, Taiyuan 030013, China)
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 21, Issue 06, 2012
DOI:
10.3760/cma.j.issn.1009-9921.2012.06.010
Key Word:
Leukemia;lymphoid;Chromosome aberrations;Gene fusion; Multiplex reverse transcriptase polymerase chain reaction

Abstract: [Objective] To investigate combined application of multiplex reverse transcription-polymerase chain reaction (mRT-PCR) and karyotype analysis detect of clonal chromosomal aberrations in acute lymphoblasfic leukemia (ALL),and explore the expression of common fusion genes.Methods 189 ALL patients were examined by multiplex RT-PCR and R or G banding techniques.[Results]10 fusion genes were detected in 69 out of 189 ALL patients(36.5%),including E2A/PBX1,TEL/AML1,BCR/ABL,MLL/AF4,MLL/AF6、MLL/AF9,MLL/AF10,MLL/ELL,SIL/TAL1,TLS/ERG.R or G banding techniques could find chromosome structural and numeracy abnormalities in 86 out of 152 patients (56.6%) available for analysis.Combination of mRT-PCR and R or G banding could raise the rate of detecting clonal chromosomal abnormalities to 69.3%.Fusion genes were detected in 33 out of 90 (36.7%) patients with adult ALL and 36out of 99 (36.4 %) patients with children ALL,there were 22 patients with positive BCR/ABL but no TEL/AML1 in adult ALL group,while there were 24 patients with positive TEL/AML.1 and 2 with positive BCR/ABL in children ALL group.There was significant statistical difference for the expression of RCR/ABL and TEL/AML1 between adult ALL and children ALL (P<0.01),but no difference for MLL related fusion gene,E2A/PBX1,SIL/TAL1 and TLS/ERG(P>0.05).BCR/ABL and TEL/AML1 fusion gene could be detected in 66 ALL patients with normal karyotype (36.3%).[Conclusion]There were different biological characteristics between adults and children with ALL.mRT-PCR technique can quickly screen chromosome structural aberrations in patients with newly diagnosed leukemia.It is useful in detection of fusion genes in ALL with normal karyotypes and it would refine the karyotype analysis and provide imrootramt prognosis-relevant information.

  • [1]Pallisgaard N,Horland P,Riishj DC,et al.Multiplex reverse transcription-polymerase chain reaction for simultaneous screening of 29 translocations and chromosomal aberrations in acute leukemia.Blood,1998,92:574-588.
  • [2]Scurto P,Hsu Rocha M,Kane JR,et al.A multiplex RT-PCR assay for the detection of chimeric transcripts encoded by the risk-stratifying transloations of pediatric acute lymphoblastic leukemia.Leukemia,1998,12:1994-2005.
  • [3]陈海敏,员海洋,樊星,等.应用多重RT-PCR在初发急性髓系白血病患者中筛查少见或难以识别的染色体异位.中国实验血液学杂志,2010,18:1138-1142.
  • [4]Yang YL,Lin SR,CherJS,et al.Multiplex reverse transcription-polymerase chain reaction as diagnostic molecular screening of 4common fusion chimeric genes in Taiwanese children with acutelymphoblastic leukemia.Pediatr Hematol Oncol,2010,32:e323-e330.
  • [5]Izraeli S,Kovar H,Gadner H,et al.Unexpected heterogeneity in E2A/PBX1 fusion messenger RNA detected by the polymerase chain reaction in pediatric patients with acute lymphoblastic leukemia.Blood,1992,80:1413-1417.
  • [6]Emst P,Fisher JK,Avery W,et al.Definitive hematopoiesis requires the mixed lineage leukemia gene.Dev Cell,2004,6:437-443.
  • [7]lsoyama K,Eguchi M,Hibi S,et al.Risk-directed treatment of infant acute lymphoblastic leukemia based on early assessment of MLL gene status:results of the Japan Infant Leukernia Study (MLL96).Br J Hematol,2002,118:999-1010.
  • [8]徐修才,胡超杰,朱薇波,等.多重巢式反转录-聚合酶链反应检测白血病相关融合基因及其临床意义.白血病·淋巴瘤,2009,18:717-720.
  • [9]葛晓燕,王宏伟,杨林花,等.急性髓系白血病混合谱系白血病基因重排的表达及临床特点.白血病·淋巴瘤,2007,16:209-211.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn