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Expression and function of chemokine CCL2 in the bone marrow cells of patients with acute lymphoblastic leukemia

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Author:
No author available
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
2
DOI:
10.3760/cma.j.issn.1009-9921.2012.02.014
Key Word:
白血病,双表型,急性;骨髓基质细胞;骨髓单个核细胞;单核细胞化学吸引蛋白质类;Leukemia,biphenotypic,acute;Bone marrow mesenchyaml cells;Bone marrow mononuclear cells;Monocyte chemoattractant proteins

Abstract: Objective To investigate the expression of CCL2 and its effects on the proliferation and adhesiveness on leukemia cells in patients with acute lymphoblastic leukemia (ALL). Methods The bone marrow mesenchymal cells (BMMSC) and bone marrow mononuclear cells (BMMNC) of 30 ALL patients and 30 healthy controls were studied, and CCL2 level was evaluated by ELISA. CCL2 gene mRNA level in ALL was determined by RT-PCR. The cell proliferation and adhesiveness were detected by MTT assays. The cell counts were measured by flow cytometry. Results The BM plasma levels of CCL2 in patients at diagnosis were significantly higher than that in healthy controls [(780.12±129.61) pg/ml vs (120.49±25.21) pg/ml,t =4.96, P =0.00]. Supernatant levels of CCL2 in BMMSC were significantly higher than that of BMMNC [(572.38±35.39) pg/ml vs (193.85±15.45) pg/ml,t =5.37,P =0.00]in vitro.CCL2 cannot induce leukemia proliferation alone,but could induce leukemia proliferation in BMMNC and BMMSC co-culture in a dose- and time-dependent manner.CCL2 could increase the leukemia adhesive to the BMMSC compared with control (r =0.824,P =0.02).Conclusion Patients with B type ALL had higher levels of CCL2 which was secreted by BMMSC. The leukemia could induce the BMMSC to secrete CCL2. CCL2 could promote the survival and proliferation of leukemia in the presence of BMMSC and BMMNC, and enhance ALL cells adhesion to BMMSC in a dose-dependent manner.

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