Effect of combination with magnetic nanoparticle of Fe3O4 and adriamycin on Raji cell line

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Author:
WANG Jing(Department of Hematology, Peking University Third Hospital, Beiiing 100191, China)
YANG Ping(Department of Hematology, Peking University Third Hospital, Beiiing 100191, China)
JING Hong-mei(Department of Hematology, Peking University Third Hospital, Beiiing 100191, China)
KE Xiao-yan(Department of Hematology, Peking University Third Hospital, Beiiing 100191, China)
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 20, Issue 12, 2011
DOI:
10.3760/cma.j.issn.1009-9921.2011.12.009
Key Word:
Magnetic nanoparticle of Fe3O4;Adriamycin;Raji cells;p53;NF-κB

Abstract: Objective To investigate the effect of combination with magnetic nanoparticle of Fe3O4and adriamycin (ADM) on Raji cell line.Methods Raji cells were cultured with Fe3O4-magnetic nanoparticle and ADM after using mechanical absortion polymerization,and the cell viability was detected by MTT and trypan-blue exclusion.The apoptosis was detected by flow cytometry,and the expression of p53 and NF-κB were measured by Western blot. Results Raji cell proliferation ratio was significantly inhibited in concentration-and time-dependent manners in both ADM and Fe3O4-MNP-ADM groups (r =0.412,P =0.027;r =0.523,P =0.014).The percentages of apoptotic cells induced by ADM and Fe3O4-MNP-ADM were 8.76 % vs 14.85 %,35.08 % vs 44.50 %,44.00 % vs 69.40 % in 12,24 and 48 hours,respectively (t =-9.137,-4.808,-6.337; P =0.012,0.041,0.024).The grey straps of NF-κB measured by Western blot were 4.22±0.32,3.31±0.28 in ADM and Fe3O4-MNP-ADM group (t =-54.416,P =0.035),whereas the grey straps of p53 were 1.042±0.114,1.270±0.091,respectively (t =33.963,P =0.047).Conclusion Combination therapy of Fe3O4-MNP and ADM could inhibit proliferation and induce apoptosis of Raji cells line,which may due to upregulation of p53 and down-regulation of NF-κB.

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