Preliminary study on thioredoxin expression in AML cells and apoptosis of AML cells induced by diamide in vitro

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Author:
XU Lian-rong(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
FENG Jiang-fang(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
NIU Bo(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
CHEN Xian-jiu(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
CHANG Bing-mei(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
XU Xian-min(Department of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China)
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 20, Issue 12, 2011
DOI:
10.3760/cma.j.issn.1009-9921.2011.12.006
Key Word:
Leukemia, experiment;Leukemia, acute;Thioredoxin;Diamide;Apoptosis

Abstract: Objective To explore the expression level of thioredoxin (Trx) in acute myeloid leukemia (AML) cells,and its association with clinical features and prognosis,as well as the effects of diamide,an inhibitor of Trx,in inducing AML cells apoptosis.Methods Expression of Trx on AML cells and fresh bone marrow cells from healthy adults were analyzed by Western-blotting. The inhibition of proliferation was measured by MTT assay.The anti-leukemia effect of diamide was observed by morphology and agarose gel electrophoresis.Results 75 % (15/20) of AML patients expressed Trx,while no expression was observed in control group.Higher expression level of Trx was associated with higher WBC counts (x2 =9.375,P < 0.05),which suggested that overexpression was associated with leukemogenesis.The inhibition of diamide on AML cells showed time and dose dependent by MTT assay.The IC50 values of diamide at 24 h,48 h and 72 h were 98.26 mg/ml,47.53 mg/ml and 8.34 mg/ml,respectively.After AML cells were treated with diamide,the apoptotic body appeared by morphology,and the typical DNA “ladder” bands were confirmed by agarose gel electrophoresis.Conclusion Trx expression level in AML cells is significantly higher than that of control group.Diamide inhibites the proliferation of AML cells by inducing apoptosis,which might be a potential agent for AML.

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