Survey on immunophenotyping analysis of acute leukemia by flow cytometry in China

( views:197, downloads:0 )
Author:
WAN Sui-gui(Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China)
LIN Yang(Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China)
XU Juan(Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China)
Journal Title:
Journal of Leukemia & Lymphoma
Issue:
Volume 20, Issue 09, 2011
DOI:
10.3760/cma.j.issn.1009-9921.2011.09.005
Key Word:
Leukemia, acute;Flow cytometry;Immunophenotypeing;Survey

Abstract: Objective To investigate the current situation of immunophenotyping of acute leukemia by flow cytometry in China (except Taiwan, Hongkong and Macau), and to provide theoretical evidence for the formulation of guidelines of immunophenotyping of leukemia by flow cytometry. Methods The Chinese Hospital Knowledge Database (CHKD) was used to retrieve the 1994-2009 published papers on immunophenotyping of acute leukemia by flow cytometry. All of 380 retrieved papers were analyzed by the GraphPad Prism 4. Results The number of published papers in China increased yearly and changed gradually from large cities to small and medium-sized cities in local distribution. The most often reported area was Beijing, followed by Jiangsu and Guangdong. In specimen processing, the ratios of using mononuclear cell labling method and whole blood labling-lysing-washing method were 34.2 % (113/330) and 65.8 % (217/330) respectively. The ratios of using single color, two color, three color and four color labling method were 15.4 % (53/344), 13.7 % (47/344), 54.1% (186/344) and 16.3 % (56/344), respectively. The number of fluorescent antibodies was used from less than 10 to more than 20. Most of them were between 11 to 16, which was 48.6 % (167/344) of total number of published papers. In data acquisition and analysis, the ratios of using FSC/SSC gating and CD45/SSC gating were 34.2 % (113/330) and 65.8 % (217/330), respectively. There were some differences in the positive criteria of lymphatic and myeloid and cytoplasm antigens. The main positive criteria of lymphatic, myeloid antigen was ≥20 %, which was 64.8 % (223/344) and 95.4 % (328/344) . The main positive criteria of cytoplasm antigen was ≥ 10 %, which accounted for 61.2 % (156/255). Conclusion A common view has been reached in specimen processing, gating method, data acquisition and analysis in immunophenotyping of acute leukemia by flow cytometry. But there were still some difference in the number of fluorescent antibodies, panels and evaluation of positive criteria of antigens.

  • [1]Hassett J,Parker J.Laboratory practices in reporting flow cytometry phenotyping results for leukemia/lymphoma specimens:results of a survey.Cytometry,1995,22:264-281.
  • [2]Homburger HA,Rosenstock W,Paxton H,et al.Assessment of interlaboratory variability of immunophenotyping: results of the College of American Pathologists Flow Cytometry survey.Ann N Y Acad Sci,1993,677:43-49.
  • [3]Kluin-Nelemans JC,van Wering ER,van't Veer MB,et al.Pitfalls in the immunophenotyping of leukaemia and leukaemic lymphomas:survey of 9 years of quality control in The Netherlands.Br J Haematol,1996,95:692-699.
  • [4]van't Veer MB,Kluin-Nelemans JC,van der Schoct CE,et el.Quality assessment of immunological marker analysis and the immunological diagnosis in leukaemia and lymphoma:a multi-centre study.Br J Hacmatol,1992,80:458-465.
  • [5]Braylan RC,Borowitz M J,Davis BH,et al.U.S.-Canadian Consensus Recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry.Cytometry,1997,30:213.
  • [6]Davis BH,Foucar K,Szczarkowski W,et al.U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry:medical indications.Cytometry,1997,30:249-263.
  • [7]Borowitz MJ,Bray R,Gascoyne R,et al.U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: data analysis and interpretation.Cytometry,1997,30:236-244.
  • [8]Stelzer GT,Marti G,Hurley A,et al.U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures.Cytometry,1997,30:214-230.
  • [9]Stewart CC,Behm FG,Carey JL et al.U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: selection of antibody combinations.Cytometry,1997,30:231-235.
  • [10]Braylan RC,Atwater SK,Diamond L,et al.U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry:data reporting.Cytometry,1997,30:245-248.
  • [11]Lanza F. Towards standardization in immunophenotyping hematological malignancies.How can we improve the reproducibility and comparability of flow cytometric results? Working Group on Leukemia Immunophenotyping.Eur J Histochem,1996,40 Suppl 1:7-14.
  • [12]Ruiz-Argtüelles A,Duque RE,Orfao A.Report on the first Latin American Consensus Conference for Flow Cytometric Immunophenotyping of Leukemia.Cytometry,1998,34:39-42.
  • [13]Rothe G,Schmitz G,Adorf D.Consensus protocols for the flow cytometric immunophenotyping of hematopoietic malignancies.Leukemia,1996,10:877-895.
  • [14]Bain BJ,Barnett D,Linch D,et al.Revised guideline on immunophenotyping in acute leukaemias and chronic lymphoproliferative disorders.Clin Lab Haematol,2002,24:1-13.
  • [15]Ruiz-Arg tü elles A,Rivadeneyra-Espinoza L,Duque RE,et al.Report on the second latin american consensus conference for flow cytometric immunophenotyping of hematological malignancies.Cytometry Part B (Clinical Cytometry),2005,70B:39-44.
  • [16]Stetler-Stevenson M,Davis B,Wood BL,et al.2006 Bethesda international consensus conference on flow cytometric immunophenotyping of hematolymphoid neoplasia.Cytometry Part B (Clinical Cytometry),2007,72B:S3.
  • [17]Wood BL,Arroz M,Barnett D,et al.2006 Bethesda international consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry:optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia.Cytometry Part B (Clinical Cytometry),2007,72B:S14-S22.
  • [18]陈珊珊.流式细胞术—白血病免疫分型规范化.中国实验血液学杂志,2002,10:1-5.
  • [19]王建中.加强临床流式细胞免疫表型分析的质量控制.中华检验医学杂志,2003,26:5-7.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn