Application of sorting peripheral blood cells by flow cytometry in the detection of peripheral Mood cell sorting

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Author:
ZHENG Mei-jing(Department of Graduate, Shanxi Medical University, Talyuan 030001, China)
DUAN Jing-jing(Department of Graduate, Shanxi Medical University, Talyuan 030001, China)
SU Li-ping()
WANG Yan-feng()
SU Wen()
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
Volume 19, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1009-9921.2010.03.010
Key Word:
Flow cytometry;T-lymphocytes;Granulocytes

Abstract: Objective To apply fluo-rescencc-activated cell sorting(FACS) in sorting T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+), which establish the separating method of series of cells from human peripheral blood, so that the scientific research and clinical research could be carried out were specifically. Methods 10 cases of normal peripheral blood were collected and T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) were stained with florescence conjugated antibodies. The positive cells were sorted by FACS. Results Before sorting the peripheral blood, the proportion of the T lymphocyte(CD_3~+) and granulocyte (CD_(15)~+) in the leukocyte is 48.8 % and 30.8 %; after sorting by FACS, the purity of T lymphocytes (CD_3~+) is up to 98 % and the recovery is about 95 %; the purity of granulocyte (CD_(15)~+) is up to 97 % and the recovery is about 96 %. Conclusion The FACS could Mlow us to quickly sort T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) with higher recovery and higher purity from the peripheral blood.

  • [1]许晓东,赵声明,郑天林,等.多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用.白血病·淋巴癌,2009,18:270-271.
  • [2]辛忠涛,薛沿宁,柳川.流式细胞分选技术在微生物表面展示文库筛选中的应用进展.微生物学免疫学进展,2003,31:62-66.
  • [3]曹云新,胡金涛,杨安钢.FACS-Aria流式细胞分选调试参数和最佳条件的设定.第四军医大学学报,2007,28:1813-1814.
  • [4]Childs R,Clave E,Contentin N,et al.Engraftment kinetics after nonmyeloablative allogeneic peripheral blood stem cell transplantation:full donor T-cell chimerism precedes alloimmune responses.Blood,1999,94:3234-3241.
  • [5]徐勇,霍梅.免疫磁珠分离及流式细胞仪分选纯化外周血CD_(34)~+/CD_(90)~+干细胞.临床检验杂志,2004,22:246-248.
  • [6]邱录贵,马月霞.造血千细胞的分离纯化与体外扩增//韩忠朝.造血干细胞理论与移植技术.郑州:河南科学技术出版社,2000:200-223.
  • [7]Zeiser R,Spyridonidis A,Watch R,et al.Evaluation of immunomodulatory treatment based on conventional and lineagespecific chimerism analysis in patients with myeloid malignancies after myelonblative allogeneic hematopoietic cell transplantation.Leukemia,2005,19:814-821.
  • [8]DeMaria MA,Zheng YL,Zhen DK,et al.Improved fetal nucleated erythrooyte sorting purity using intracellular antifetal hemoglobin and Hoechst 33342.Cytometry,1996,25:37-45.
  • [9]Shindler KS,Roth KA.Cholera toxin binds to differentiating neurons in the developing murine basal ganglia.Brain Res Dev Brain Res,1996,92:199-210.
  • [10]Rens W,Welch GR,Houck DW,et al.Slit-scan flow cytometry for consistent high resolution DNA analysis of X-and Y-chromosome bearing sperm.Cytometry,1996,25:191-199.
  • [11]Kaprelyants AS,Mukamolova GV,Davey HM,et al.Quantitative analysis of the physiological heterogeneity within starved cultures of micrococcus luteus by flow cytometry and cell sorting.Appl Environ Microbiol,1996,62:1311-1316.
  • [12]Van Dilla MA,Deaven LL.Construction of gene libraries for each human chromosome.Cytometry,1990,11:208-218.
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