The value of flow cytometry in diagnosis and subclassification of non-Hodgldn lymphoma

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Author:
HUI Wu-han(Department of Hematology, Capital Medical University Xuanwu Hospital, Beijing 100053, China)
XU Juan(Department of Hematology, Capital Medical University Xuanwu Hospital, Beijing 100053, China)
SUN Xue-jing(Department of Hematology, Capital Medical University Xuanwu Hospital, Beijing 100053, China)
WAN Sui-gui(Department of Hematology, Capital Medical University Xuanwu Hospital, Beijing 100053, China)
SU Li(Department of Hematology, Capital Medical University Xuanwu Hospital, Beijing 100053, China)
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
Volume 18, Issue 10, 2009
DOI:
10.3760/cma.j.issn.1009-9921.2009.10.006
Key Word:
Lyrnphoma, non-Hodgkin; Flow cytometry; Immunotype

Abstract´╝Ü Objective To evaluate the utility of flow cytometry (FCM) in diagnosis and subclassification of non-Hodgkin lymphoma (NHL). Methods The samples of lymph nodes biopsy from 59 cases clinically suspected of NHL were detected by flow cytometry; and clonal lymphocytes and their immunophenotypes were identified analyzed. The concordance between the results of flow cytometry and histopathology was analyzed. Results Among the 59 cases, flow cytometry was able to identify aberrant clonal lymphocytes in 24 of 28 NHL cases identified by histopathology, the neoplastic lymphocytes ranged from 4.28 % to 89.10 %; 23 cases were diagnosed as B-NHL and 1 case was diagnosed as T-NHL. Compared with histopathology, the accuracy of FCM was 85.71% in diagnosis of NHL. The specificity and sensitivity of FCM was 100 % and 92% in diagnosis of B-NHL. The accuracy of flow cytometry immunophenotyping in classification of 24 cases of NHL was consistent with that of histopathology. Conclusion Flow cytometry could be an ancillary technique in diagnosis of NHL by identifying aberrant clonal lymphocytes, and enable identification of B-NHL subtype.

  • [1]Craig FE,Foon KA.Flow cytometric immunophenotyping for hematologic neoplasms.Blood,2008,111:3941-3967.
  • [2]Gorczyca W,Weisberger J,Liu Z,et al.An approach to diagnosis of T-cell lymphoproliferative disorders by flow cytometry.Cytometry,2002,50:177-190.
  • [3]Fiona E.Craig.Flow cytometric evaluation of B-cell lymphoid neoplasms.Clin Lab Med,2007,27:487-512.
  • [4]Kaleem Z,White G,Vollmer RT.Critical analysis and diagnostic usefulness of limited immunophenotyping of B-cell non-Hodgkin lymphomas by flow cytometry.Am J Clin Pathol,2001,115:136-142
  • [5]Kaleem Z,Zehnbauer BA,White G,et al.Lack of expression of surface immunoglobulin light chains in B-cell non-Hodgkin lymphomas.Am J Clin Pathol,2000,113:399-405.
  • [6]Beck RC,Stahl S,O'Keefe CL,et al.Detection of mature T-cell leukemias by flow cytometry using anti-T-cell receptor V beta antibodies.Am J Clin Pathol,2003,120:785-794.
  • [7]Karube K,Aoki R,Nomura Y,et al.Usefulness of flow cylometry for differential diagnosis of precursor and peripheral T-cell and NK-cell lymphomas:analysis of 490 cases.Pathol lnt,2008,58:89-97.
  • [8]lnaba T,Shimazaki C,Sumikuma T,et al.T-cell associated antigen-positive B-cell lymphoma.Leuk Lymphoma,2001,42:1161-1171.
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