Changes of the expression level of Par-4 and WT1 genes during K562 cell apoptosis induced by arsenic trioxide

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Author:
QIN Jie(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
WANG Hong-wei(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
YANG Tao(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
ZHU Lei(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
XU Yong-qun(Department of Postgraduate, Shanxi Medical University, Taiyuan 030001, China)
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
Volume 18, Issue 05, 2009
DOI:
10.3760/cma.j.issn.1009-9921.2009.05.002
Key Word:
Arsenicals; K562 cells; Apoptosis; Tenes, Par-4; Genes, Wilms tumor

Abstract: Objective To explore the changes of Par-4 and WT1 genes expression during human leukemic K562 cells apoptosis induced by arsenic trioxide (As2O3). Methods After the K562 cells were treated with arsenic trioxide (2-10 μmol/L) for 24-72 hours, cell survival rate was evaluated by MTT assay and apoptosis was analyzed using flow cytometry. The expressions of mRNA and protein for par-4 and WT1 were tested by Real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) method and Western blotting in K562 cells with various concentrations of arsenic trioxide at different time points. Results After the K562 cells were treated with As2O3 of different concentrations, arsenic trioxide could efficiently inhibit the growth of K562 cells and induce apoptosis with the increase of As2O3 concentration. The same time, the mRNA and protein expressions of Par-4 were up-regulated, while the mRNA and protein expression of WT1 were down-regulated. Conclusion Arsenic trioxide could inhibit the growth of K562 cells and induce apoptosis. Par-4 and WT1 genes could participate in the apoptosis of K562 cells induced by arsenic trioxide.

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