Effect of stromal cell-derived factor-1 on the adherence function of mononuclear cells
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2008.03.005
- Key Word:
- 胎血;细胞黏附;基质细胞衍生因子-1;迟现抗原-4;Fetal blood;Adherence;Stromal cell-derived factor-1;Very late antigen-4
Abstract： Objective To investigate the effects of stromal cell-derived factor-1 (SDF-1) on the adherence of mononuclear cells (MNC). Methods Mononuclear cells were isolated from cord blood and the adherent rate was assayed by using MTT with fibronectin coated in different concentration of SDF-1. Then the expression of adherent molecule VLA-4 by FACS as well as the adherent rate when MNC were cultured in vitro. Results The adherent rate of fresh cord blood MNC was (110.2±2.10) %, (128.3±2.09) %, (141.6±2.62) %, (160.8±3.84) %, (162.3±3.60) %, (165.4±2.73) %, (165.1±2.33) %. When the mass concentration of SDF-1 was 50, 100, 150, 200, 250 ng/ml. When MNC were cultured in vitro,the expression of VLA-4 was 26.37te was (160.2±6.38) %, (194.6±6.09) %, (147.5±6.86) %, (144.7±4.96) %, (118.1±3.64) %. The correlation coefficient between the adherent rate and the expression of VLA-4 is 0.826. Conclusion The adherent rate of fresh cord blood MNC increases along with the concentration of SDF-1, however, it tends to be stable when the concentration of SDF-1 reaches 100 ng/ml. When MNC were cultured in vitro with hemopoietic growth factors, the expression of VLA-4 and the adherent rate of MNC increased in the initial stage, however, the expression of VLA-4 and the adherent rate of MNC both decreased gradually along with time extending. There is positive correlation between the adherent rate of MNC and the expression of adherent molecule VLA-4.
- [1]莫文健,毛平,何秋山,等.无血清脐血巨核系祖细胞体外扩增的研究[J].2004,(2).
- [2]张洁霞,毛平.脐血CD34+细胞及红系祖细胞扩增的实验研究[J].2005,(3).
- [3]李桥川,李云涛,孟恒星,等.基质细胞衍生因子-1和血小板第4因子对体外扩增后脐血CD34+细胞黏附特性和趋化功能的影响[J].2006,(1).
- [4]M A, Dao,A J, Shah,G M, Crooks,etc.Engraftment and retroviral marking of CD34+ and CD34+CD38- human hematopoietic progenitors assessed in immune-deficient mice.[J].1998,91(4).
- [5]W, Piacibello,F, Sanavio,L, Garetto,etc.Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood.[J].1997,89(8).
- [6]C. DE BRUYN,A. DELFORGE,D. BRON,etc.Ex Vivo Expansion of CD34+CD38- Cord Blood Cells[J].1997,6(2).
- [7]Springer T,Webb IJ,Bleul C,etc.The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood.[J].1997,185(1).
- [8]G, Capmany,S, Querol,J A, Cancelas,etc.Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells.[J].1999,84(8).
- [9]Azza A, Mohamed,Azza M, Ibrahim,Manal W, El-Masry,etc.Ex vivo expansion of stem cells: defining optimum conditions using various cytokines.[J].2006,12(2).
- [10]Ploemacher RE,Breems DA,Blokland EA.Stroma-conditioned media improve expansion of human primitive hematopoietic stem cells and progenitor cells.[J].1997,11(1).
- [11]K M, Luens,M A, Travis,B P, Chen,etc.Thrombopoietin, kit ligand, and flk2/flt3 ligand together induce increased numbers of primitive hematopoietic progenitors from human CD34+Thy-1+Lin- cells with preserved ability to engraft SCID-hu bone.[J].1998,91.
- [12]Y, Yonemura,H, Ku,S D, Lyman,etc.In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand.[J].1997,89(6).