Preliminary report of expression of hepcidin gene in Wistar rats ACD model is affected by EPO
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2008.03.002
- Key Word:
- 慢性病性贫血;hepcidin mRNA;红细胞生成素;铁代谢;反转录-聚合酶链反应;Anemia in chronic disease;hepcidin mRNA;Erythropoietin;Iron metabolism;RT-PCR
Abstract： Objective To investigate the expression changes of the liver hepciding gene in the formation process of Wistar male rats anemia in chronic disease(ACD) model. Methods ACD rat model was established by repeated injection of Freund' s complete adjuvant. The relationship between hepcidin mRNA expression levels of normal rats, ACD rats and ACD rats after intra -peritoneal injection of EPO. Results Anemia was induced in the rat model. In the ACD group, hepcidin mRNA expression levels increased; after intra-peritoneal injection of EPO, hepcidin mRNA expression levels decreased, anemia is improved. Conclusion Hepcidin plays an important role in pathogenesis of ACD. EPO could be decreased hepcidin mRNA expression levels by anti- inflammatory reaction and decrease cell factors. Decrease of hepcidin level as early as possible will benefit to block the development of anemia, that will provide a new strategy of therapy for ACD.
- [1]Means RT.Hepcidin and cytokines in anaemia.[J].2004,9(5).
- [2]Elizabeta, Nemeth,Erika V, Valore,Mary, Territo,etc.Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein.[J].2003,101(7).
- [3]Kristal B,Shasha SM,Shapiro G,etc.Interaction between Erythropoietin and Peripheral Polymorphonuclear Leukocytes in Continuous Ambulatory Dialysis Patients.[J].2002,91(4).
- [4]Vardar E,Gokmen N,Yilmaz O,etc.Recombinant human erythropoietin counteracts secondary injury and markedly enhances neurological recovery from experimental spinal cord trauma.[J].2002,99(14).
- [5]李蓉生.应重视慢性病贫血的诊断和治疗[J].1999,(11).
- [6]Ilyin G,Pigeon C,Leroyer P,etc.A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload.[J].2001,276(11).
- [7]Grandchamp B,Beaumont C,Vaulont S,etc.Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice.[J].2001,98(15).
- [8]Weinstein DA,Roy CN,Andrews NC.2002 E. Mead Johnson Award for Research in Pediatrics Lecture: the molecular biology of the anemia of chronic disease: a hypothesis.[J].2003,53(3).
- [9]W C, Faquin,T J, Schneider,M A, Goldberg.Effect of inflammatory cytokines on hypoxia-induced erythropoietin production.[J].1992,79(8).
- [10]Aydemir F,Knutson MD,Koss LM,etc.Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin.[J].2005,102(5).
- [11]Park CH,Valore EV,Waring AJ,etc.Hepcidin, a urinary antimicrobial peptide synthesized in the liver.[J].2001,276(11).
- [12]Gelbart T,Lee P,Wang L,etc.Regulation of hepcidin transcription by interleukin-1 and interleukin-6.[J].2005,102(6).
- [13]Bigard X,Nicolas G,Viatte L,etc.The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation.[J].2002,110(7).