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Primary study of the phenotype and biological characteristics of chronic myelogenous leukemia derived mesenchymal stem cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2008.02.003
- Key Word:
- 白血病,髓样,慢性;间质干细胞;融合蛋白质类,bcr-abl;Leukemia,myeloid,chronic;Mesenchymal stem cell;Fusion proteins,bcr-abl
Abstract： Objective To isolate and study the phenotype and biological characteristics of chronic myelogenous leukemia-derived mesenchymal stem cells. Methods Cultivate the mesenchymal stem cells from chronic myelogenous leukemia in vitro, identify the immunophenotype by fluorescence-activated cell sorter (FACS), and detect the bcr-abl fusion gene by RT-PCR and FISH. Results The mesenchymal stem cells from chronic myelogenous leukemia can express the bcr-abl fusion gene. Conclusion These findings provide the evidence that the rearrangement of bcr-abl gene might happen at least more primitive than CD+34 CD-38 cells.
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