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Expression and mutation analysis of the tumour suppressor gene Rox in leukemia cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.06.004
- Key Word:
- 基因,肿瘤抑制;基因,Rox;白血病;突变
Abstract： 目的 分析Rox基因突变在白血病发病中的作用.方法 利用反转录-聚合酶链反应(RT-PCR)、单链构象多态性(SSCP)分析及DNA序列分析技术,检测26例初治急性白血病(AL)患者、30名正常健康志愿者和7种白血病细胞系Rox基因表达及突变情况.结果 RT-PCR显示所有标本中均可检测到Rox基因的表达,在3例初治AL患者发现3处错义突变,突变率为11.5%.结论 首次在AL中发现Rox基因突变,提示Rox基因的突变可能与AL的发病有关.
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