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Separation of endothelial cell from lymph node of lymphoma using laser capture microdissection
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.05.003
- Key Word:
- 淋巴瘤;细胞;内皮;血管
Abstract： 目的 探讨淋巴瘤发生的分子机制及有关的基因改变,研究分离、分析淋巴结中血管内皮细胞基因表达差异的方法.方法 将淋巴瘤淋巴结标本液氮速冻,冷冻切片,快速免疫组化标记血管内皮细胞,使用激光显微切割技术分离血管淋巴细胞,提取血管内皮细胞中的mRNA,使用RT-PCR检测所得RNA的质量.结果 成功地实现了血管内皮细胞的分离,并保持了血管内皮细胞中RNA的完整性.结论 确立了一个适于分离淋巴瘤血管内皮细胞的激光显微切割方法.
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