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iASPP oncoprotein potentates the proliferation peculiarity of cells derived from murine bone marrow
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.05.002
- Key Word:
- 癌基因蛋白质类;iASPP;转导;遗传;反转录病毒
Abstract： 目的 研究iASPP(一个新发现的p53家族抑制性成员)在小鼠骨髓细胞中过表达对细胞增生作用的影响.方法 构建反转录病毒载体,通过基因转导方法,使小鼠骨髓细胞过表达人类iASPP或(和)突变的N-Ras,采用集落形成实验检测细胞体外增生能力的变化.结果 转导iASPP的小鼠骨髓细胞与转导空载体对照组相比集落形成能力显著增强(P＜0.01),转导突变的N-Ras也能提高小鼠骨髓细胞的体外增生能力,二者差异无统计学意义(P＞0.05),但iASPP联合突变的N-Ras可使集落的数量显著多于单基因组(P＜0.05)及空载体对照组(P＜0.01),而且集落体积增大.结论 癌蛋白iASPP可以显著提高小鼠骨髓细胞的体外增生能力,并且与突变的N-Ras具有协同作用,因而iASPP有望成为研究肿瘤及白血病治疗的新靶点.
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