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Inhibition effect of antisense Bmi-1 on Raji cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.05.001
- Key Word:
- Raji细胞;反义Bmi-1;白血病;基因;p16
Abstract： 目的 观察反义Bmi-1对B淋巴细胞白血病Raji细胞的抑制作用并探讨其机制.方法 在体外转染中通过阳离子脂质体法将反义质粒导入Raji细胞中,以G418进行筛选得到阳性克隆;以MTT法和体外集落形成实验检测反义Bmi-1表达质粒对Rajii细胞增生的抑制作用;利用流式细胞仪分析细胞周期;采用免疫荧光组化法检测反义Bmi-1对Raji细胞的p16蛋白的上调作用.结果 转染反义Bmi-1表达质粒组与对照组(空白对照及空质粒组)相比较,生长速度明显变慢,转染反义Bmi-1表达质粒组与对照组(空白对照及空质粒组)相比较,生长速度明显变慢,克隆形成能力明显下降,空白对照组细胞集落数为(89.7±8.07)/103细胞,空质粒组细胞集落数为(81.3±6.91)/103细胞,转染反义质粒组细胞集落数为(50.0±5.21)/103细胞(P＜0.01);与对照组细胞相比反义组G1期所占比例明显上升(P＜0.05);p16蛋白表达也明显增强.结论 反义Bmi-1对Raji细胞的体外生长具有明显的抑制作用.
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