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Establishment and application of a murine model for bone marrow transplantation following purging of acute myeloid leukemia cells in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.04.004
- Key Word:
- 白血病,粒细胞,急性;净化;骨髓移植
Abstract： 目的 建立一个小鼠急性髓系白血病(AML)净化后骨髓移植的模型并探讨益康唑(Econazole,Ec)药物净化的效果.方法 利用小鼠P815 AML细胞转染新霉素抗性基因,即NeoR基因后与小鼠骨髓细胞混合,体外短期培养净化;通过移植后血液系统恢复,白血病微小残留病变和小鼠的存活进行评估.结果 接受放射治疗后经尾静脉注射P815/NeoR细胞而未经净化的小鼠100%可检测到白血病细胞;Ec体外净化后骨髓移植小鼠造血恢复没有延迟,而残留白血病细胞阳性率明显降低且小鼠的总生存明显改善.结论 实验所设计的P815/NeoR小鼠AML净化后骨髓移植模型简单易行、重复性好;Ec可用于AML的净化后骨髓移植.
- [1]张翼鷟,张伯龙,姚善谦,等.自体外周血造血干细胞和自体骨髓移植治疗急性髓性白血病的临床疗效比较研究[J].2003,(1).
- [2]M.K Brenner,D.R Rill,R.A Krance,etc.Gene-marking to trace origin of relapse after autologous bone-marrow transplantation[J].1993,341(8837).
- [3]N C, Gorin,P, Aegerter,B, Auvert,etc.Autologous bone marrow transplantation for acute myelocytic leukemia in first remission: a European survey of the role of marrow purging.[J].1990,75(8).
- [4]G, Meloni,D, Diverio,M, Vignetti,etc.Autologous bone marrow transplantation for acute promyelocytic leukemia in second remission: prognostic relevance of pretransplant minimal residual disease assessment by reverse-transcription polymerase chain reaction of the PML/RAR alpha fusion gene.[J].1997,90(3).
- [5]B Douglas, Smith,Richard J, Jones,Shing M, Lee,etc.Autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide purging for acute myeloid leukaemia beyond first remission: a 10-year experience.[J].2002,117(4).
- [6]Philip, Thierry,Guglielmi, Cesare,Hagenbeek, Anton,etc.Autologous Bone Marrow Transplantation as Compared with Salvage Chemotherapy in Relapses of Chemotherapy-Sensitive Non-Hodgkin's Lymphoma.[J].1995,333(23).
- [7]Papa G,de Witte T,Labar B,etc.Autologous or allogeneic bone marrow transplantation compared with intensive chemotherapy in acute myelogenous leukemia. European Organization for Research and Treatment of Cancer (EORTC) and the Gruppo Italiano Malattie Ematologiche Maligne dell'Adu[J].1995,332(4).
- [8]Crump M,Berge SA,Zhang Y.Purging of contaminating breast cancer cells from hematopoietic progenitor cell preparations using activation enhanced cell death.[J].2002,72(3).
- [9]Ifrah N,Attal M,Bataille R,etc.A prospective, randomized trial of autologous bone marrow transplantation and chemotherapy in multiple myeloma. Intergroupe Francais du Myelome.[J].1996,335(2).
- [10]Blaise D,Gorin NC,Labopin M,etc.Autologous blood cell vs marrow transplantation for acute myeloid leukemia in complete remission: an EBMT retrospective analysis.[J].2000,25(11).